小鼠移植心脏保存极限的研究

Ultimate cold preservation time in Ringer's solution for murine cardiac graft

目的 :确定小鼠心脏移植的供心在体外 4℃林格氏液中保存时间的极限。方法 :小鼠供心分别于体外保存 2、4、6、8、10h ,而后进行腹部异位心脏移植 ,观察血供接通后移植心脏的复跳率及移植物 1周存活率 ,并于移植后 2 4h及 1周检测移植物形态学变化及中性粒细胞、T细胞、巨噬细胞浸润情况 ,细胞间粘附分子ICAM - 1的表达强度。结果 :小鼠供心在 4℃林格氏液中保存 6h后 ,复跳率达 10 0 % (6 / 6 ) ,移植物 1周存活率 83 3% (5 / 6 ) ,组织形态、细胞浸润情况及ICAM - 1表达强度与无保存对照组之间无明显差异。结论 :小鼠供心在林格氏液中的保存极限为 6h 。

AIM: To determine the ultimate preservation time of murine cardiac grafts in 4℃ Ringer's solution. METHODS: Murine cardiac grafts were implanted to the abdominal vessels heterotopically 2, 4, 6, 8 and 10 hours after cold preservation. Graft survival rate and histological morphological changes, as well as the neutrilphil, T cell, macrophage infiltration, ICAM expression were determined by immunohistochemistry. RESULTS: The cardiac function-recovery rate and 1-week graft survival rate were 100% and 83.3% in 6-hour preservation group. Compared with non-preservation control group, no more apparent histological damages, cell infiltration and ICAM expression were found. CONCLUSION: The ultimate preservation time of murine cardiac graft in 4℃ Ringer's solution was 6 hours. [