HPLC快速测定鸡肉中二氯二甲吡啶酚残留

Rapid Determination of Clopidol Residue in Chicken Muscle by HPLC

本实验研究了一种快速、简便的测定鸡肉中二氯二甲吡啶酚(Clopidol)残留量的高效液相色谱法。本法采用微量化技术处理样品,以乙腈-氯仿为提取剂提取样品中残留的二氯二甲吡啶酚,提取液经挥发干后,在正已烧和硫酸钠水溶液两相间进行分配以去除脂类物质,然后再用乙腈-氯仿从水中将二氯二甲吡啶酚提取出来,将有机相挥发至干,残渣定量溶解于流动相中,以RP-HPLC分离,紫外检测器于265nm波长下检测,外标法定量。空白鸡肉样品添加0.01×10^(-6)～0.20×10^(-6)二氯二甲吡啶酚,其回收率为73.4%～108.6%,变异系数为6.3%～8.1%。方法检测限可达5μl/kg(5g样品,最后定容0.5ml,进样20μl),可满足鸡肉中二氯二甲吡啶酚残留的准确、快速测定。

The residue contaning clopidol obtained from chicken muscle by pretreatment was dissolvedin moble phase and then eluaed with a solution of acetonitrile-acetic acid-water (4.0+0.5+95.5) and detected at 265 nm. The method have a recovery range of 73.41%～08%, adetection limit of 5×10^(-6) and a coefficient of variation of 6.3%.