前列腺增生症所致膀胱出口梗阻患者逼尿肌中神经生长因子mRNA的变化及意义

Change of nerve growth factor mRNA in human detrusor in bladder outlet obstruction with benign prostatic hyperplasia and their implication

目的 探讨前列腺增生症 (BPH)所致膀胱出口梗阻 (BOO)患者膀胱逼尿肌中神经生长因子 (NGF)mRNA的表达变化及意义。方法 对同龄对照组 8例膀胱癌患者、BPH梗阻逼尿肌稳定组 2 4例患者和BPH梗阻逼尿肌不稳定组 16例患者的膀胱壁逼尿肌组织 ,采用逆转录聚合酶链反应(RT PCR)检测膀胱逼尿肌细胞中NGFmRNA的表达。结果 NGFmRNA在同龄对照组、BPH梗阻逼尿肌稳定组和梗阻逼尿肌不稳定组患者的膀胱逼尿肌细胞中均有表达 ,三组患者的平均表达水平两两之间差异均有显著性意义 (P <0 0 1) ,而且NGFmRNA在同龄对照组、BPH梗阻逼尿肌稳定组和梗阻逼尿肌不稳定组的平均表达水平逐渐增加。结论 前列腺增生症引起膀胱出口梗阻后 ,膀胱逼尿肌细胞中NGFmRNA表达水平增高 ,并可能与逼尿肌不稳定 (DI)的发生有关。

Objective To investigate the change of nerve growth factor (NGF) mRNA in human detrusor in bladder outlet obstruction (BOO) with benign prostatic hyperplasia (BPH) and their implication Methods Eight cases of bladder cancer and 40 patients with BPH were included in this study All patients were divided into three groups,a control group,an obstructive detrusor stability group and an obstructive detrusor instability group NGF mRNA in detrusor from all patients was measured using a RT PCR Results The RT PCR study indicated that NGFmRNA was expressed in detrusor of three groups of patients There were significant differences among the three groups( P <0 01) The expression of NGF mRNA in the obstructive instability group was higher than that in the obstructive stability group and in the control group The NGF mRNA level in the obstructive stability group was higher than that in the control group Conclusion The expression of NGF mRNA in detrusor was elevated in BOO with BPH The elevated expression of NGF mRNA might be correlated with detrusor instability (DI) due to BOO