基因芯片检测结核分枝杆菌利福平和异烟肼耐药性研究

The use of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin and isoniazid

目的 研究基因芯片在检测结核分枝杆菌利福平和异烟肼耐药性中的应用。方法根据与利福平和异烟肼耐药相关的 4条基因上的 11个位点的 30种单核苷酸多态性设计制作芯片 ,用芯片检测结核分枝杆菌的基因突变 ,从而判断其耐药性。结果 用基因芯片在 110株异烟肼耐药株中检测出 85株 (73 3% ) ,在 30株异烟肼敏感株中检测出 2 2株 (73 3% ) ,在 94株利福平耐药株中检测出 77株 (81 9% ) ,在 4 6株利福平敏感株中检测出 4 0株 (87 0 % )。芯片检测结果与部分样本的测序结果完全相符。结论 用DNA芯片检测结核分枝杆菌对利福平和异烟肼的耐药性具有较高的特异性和敏感性 ,该法快速、精确 ,可以应用于结核分枝杆菌耐药性的检测。

Objective To study the application of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin (RFP) and isoniazid (INH). Methods Probes were designed and the gene chip was fabricated according to the 30 single nucleotide polymorphisms of 11 mutations on 4 genes associated with RFP and INH resistance. The mutations in Mycobacterium tuberculosis were detected by gene chip to analyze the resistance to INH and RFP. Results 85 of 110 (77.3%) strains resistant to INH and 22 of 30 (73.3%) strains sensitive to INH were detected, while 77 of 94 (81.9%) strains resistant to RFP and 40 of 46 (87.0%) strains sensitive to RFP were detected. The results from the gene-chip detection were consistent with the sequence information. Conclusion The gene-chip technology, a fast test with high accuracy, specificity and sensitivity, as shown in our study, is promising in the clinical detection of Mycobacterium tuberculosis resistant to INH and RFP.