高效液相色谱法测定人胎盘中的多胺

Quantation of Polyamine in Human Placent by High-performance Liquid Chromatography

本文研究了一种简单而又快速的处理人胎盘的方法。用苯甲酰氯进行柱前衍生,衍生的多胺用反相高效液相色谱进行分离分析,最佳的衍生试剂的用量是7μl,衍生温度为36.5℃。分析表明,老化胎盘中的精脒、精胺的含量比正常胎盘的要高得多,它们之间的差异非常显著;而正常胎盘中三种多胺含量和钙化胎盘的相比确无明显差异。这一结论可为临床检验胎盘老化与否提供科学依据。

The separation and the determination of polyamine in human placent by HPLC were described. The analytical result shows that the levels of putrescine. spermidine and spermine in the normal placent were less than those in the ageing one ,the difference between them is very remarkable, but the difference of the levels of the three polyamine (putrescine, spermidine and spermin) between normal and calcification was not significant. The conclusion provides sciectific basis for testing whether the placent is ageing or not in clinical.