肝素酶mRNA在胃癌组织中的表达及其与c-met表达的关系

Increased expression of heparanase mRNA is correlated with metastasis of gastric carcinoma and is probably correlated with c-met protein

目的 研究胃癌组织中肝素酶mRNA的表达与胃癌临床病理特征及肿瘤微血管密度之间的关系 ,并探讨胃癌组织中的肝素酶表达增高的机制。方法 采用逆转录 -聚合酶链反应(RT PCR)法检测 4 7例手术切除的胃癌组织及癌旁组织中肝素酶mRNA的表达 ,免疫组织化学法检测c met在胃癌组织中的表达 ,CD34免疫组织化学染色 ,计数肿瘤微血管密度。结果 胃癌组织中肝素酶表达阳性率明显高于相应的癌旁组织 (74 5 %vs 19 1% ,P <0 0 1)。肝素酶表达阳性组的胃癌组织 ,其微血管密度显著高于阴性组 (5 1± 15vs 31± 10 ,P <0 0 1)。肝素酶mRNA的表达与胃癌患者的肿瘤直径、淋巴结转移、浸润深度以及TNM分期有关 ,与胃癌患者的年龄、性别、分化程度及有无远处转移无关。胃癌组织中c met阳性率为 85 1% ,与肝素酶mRNA表达之间的相关系数为 0 714 (P <0 0 1)。结论 胃癌组织中肝素酶mRNA表达水平增高 ,肿瘤微血管密度增高 ,促进了胃癌的生长、浸润及转移 ,与胃癌预后不良的临床病理特征有关。c met癌基因可能参与了肝素酶的表达调控。

ObjectiveTo explore the expression of human heparanase mRNA and its relationship with the clinicopathological characteristics and angiogenesis and its possible mechanism in gastric carcinomas. MethodsExpression of heparanase mRNA in gastric cancerous tissues and its corresponding adjacent tissues were detected by RT-PCR in forty-seven patients. Expression of c-met protein and microvessel density (MVD) were examined by immunohistochemcal staining. ResultsExpression of heparanse mRNA was significantly higher in gastric cancerous tissues than in its adjacent tissues (74.5% vs. 19.1%,P<0.01). MVD was significantly higher in heparanase positive group than its counterpart (51±15 vs. 31±10,P<0.01). Expression of heparanase mRNA was correlated with expression of c-met (r=0.714,P<0.01). The positive rate of heparanse expression in gastric carcinomas were correlated with tumor diameter,invasive depth,regional lymph nodes metastasis and TNM stage,whereas there were no relation with gender,age,tumor differentiation and remote metastasis of the patient. ConclusionIncreased heparanase expression contributes to metastasis and angiogenesis in gastric cancer. Heparanase mRNA expression correlates with poor clinicopathologic characteristics in its prognosis. C-met is correlated with heparanase expression.