肺腺癌患者酪氨酸激酶信号传导通路的异常与临床预后

The abnormity and clinical value of tyrosine kinase signaling pathway in lung adenocarcinoma

目的 探讨肺腺癌患者酪氨酸激酶传导通路的异常和临床预后的关系。方法 抽提10例肺腺癌标本及其癌旁组织的总RNA ,标记荧光 ,与含 1382 4个基因的基因芯片进行杂交 ,经洗片、扫描 ,计算机分析比较两种组织的差异表达基因。每次实验重复 2次 ,结果取平均值。采用聚类分析和判别分析对海量数据进行数据挖掘 ,得出结果后再与临床预后因素结合分析。结果 根据酪氨酸激酶传导通路上的差异 ,10例标本聚类为 3类 ,海量基因聚为 5类。不同预后的标本有不同的特征基因群 ,预后最好的一组患者高表达抗增殖和免疫相关基因 ;预后最差的患者在高表达与增殖相关基因的同时 ,抗增殖和免疫防御相关基因处于低表达水平。结论 肺腺癌的酪氨酸激酶传导通路异常主要表现为 3种类型 ,由此决定了肺腺癌不同的生物学行为和不同的预后。

ObjectiveTo study the abnormity and clinical value of tyrosine kinase signaling pathway in lung adenocarcinoma. MethodsTotal RNA was extracted form 10 lung adenocarcinoma samples and matched normal tissues. cDNA was labeled fluorescence during reverse transcription and hybridized on 20 slides of microarray with 13 824 genes. After washed and scanned,the cy3:cy5 ratios were obtained by computer analysis. Each experiment was repeated two times and the mean value was chosen. Cluster analysis and discriminant analysis were used to mine the pool data. The results were combined with clinical prognostic factors for further analysis. ResultsAccording to the abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma,sample cluster divided the 10 samples into 3 groups and gene cluster divided all genes into 5 groups. Different prognosis samples had different characteristic genes. The patients with the best prognosis overexpressed some genes related to immunity and inhibition proliferation,and those with the worst prognosis overexpressed some genes related to proliferation while the genes related to immunity and inhibition proliferation were expressed at a low level. ConclusionThere are 3 type abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma that are related to different biological behavior and different prognosis.