摘要
目的 :在嵌合HAb18 Fab抗体的基础上 ,利用载体pComb3X ,采用导向选择链更替技术建立全人源性Fab基因库。方法 :用RT PCR自肝癌患者外周血单个核细胞 (PBMC)中 ,扩增全套人抗体Fd基因片段和L链基因。首先 ,将Fd基因克隆入已含嵌合L链基因的展示载体pComb3X/CL中 ,建立人 -鼠杂合的Fab基因库 ,以原核表达的非融合HAb18G的胞外区片段为抗原 ,筛选杂合的Fab基因 ,以得到人Fd基因。然后应用筛选出的Fd基因与人L链基因库配对 ,建立全为人Fab的基因库 ,并筛选全人Fab基因。将IPTG诱导的表达菌裂解 ,取其上清对pⅢ Fab融合蛋白的功能进行分析 ,并对其编码基因进行测序。结果 :建立了库容为 2× 10 7的杂合Fab基因库 ,经 6轮筛选后得到 7株杂合Fab基因。利用筛选的人Fd基因建立了库容为 0 .8× 10 7的全人Fab基因库 ,经 4轮筛选得到 2株亲和力较高、特异性强的人Fab基因。测序结果显示 ,2株Fab基因具有相同的Fd基因序列 ,与亲本抗体同属IgG2亚类 ;L链基因为κ型 ,可变区均属于Vκ3家族。结论 :利用导向选择链更替技术 ,筛选出特异性较强、完全人源化的抗肝癌抗原HAb18G的Fab抗体 ,为进一步的工作打下了基础。
AIM: To prepare humanized Fab antibody by guided selection and chain shuffling technique. METHODS: Human Fd and C L repertoire genes were amplified by RT-PCR from PBMCs of patients with hepatocellular carcinoma. Human-murine chimeric C L genes were paired with the human Fd repertoire genes to construct phage antibody library. After four rounds of panning against HAb18GE, chimeric antibodies containing humanized Fd gene and binding to HAb18GE were obtained. Then the selected Fd genes were paired with the human C L repertoire genes to construct a humanized Fab antibody gene library. After four rounds of panning, completely humanized Fab antibodes were obtained. RESULTS: After six rounds of panning, 7 chimeric Fab genes were obtained from the Fab gene library with 2×107 pfu. Using the selected seven Fd genes, the humanized Fab gene library of 0.8×107 pfu was constructed. After four rounds of panning, 2 humanized Fab genes with the strongest reactivity to HAb18G were obtained. DNA sequencing showed that Fds of the two humanized Fabs had the same DNA sequence, which belonged to IgG2, just the same as the parent antibody. And the C L belonged to kappa type, and Vκ3 family. CONCLUSION: By guided-selection and chain shuffling technique, the humanized Fab antibody against HAb18G is obtained, which lays the foundation for further research.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第4期437-440,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目 (No .30 0 70 842 )
关键词
抗体基因库
人源化
肝癌
pComb3X
导向选择
链更替
antibody library
humanization
hepatocellular carcinoma
pComb3X
guided selection
chain shuffling