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大鼠外周血内皮祖细胞体外分离鉴定及内皮细胞的分化 被引量：3

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摘要 目的 :建立一种稳定的体外分离培养及诱导分化大鼠外周血内皮祖细胞 (EPC)的方法。方法 :抽取SD大鼠外周血 ,应用Ficoll密度梯度离心的方法获得单个核细胞 ,同时应用VEGF和bFGF诱导 ,使之向内皮细胞分化 ,以免疫细胞化学鉴定贴壁细胞的内皮标志CD31,CD34,Flk 1和vWF。结果 :经VEGF和bFGF诱导后的EPC的内皮标志CD31,CD34,Flk 1和vWF在不同时段呈阳性表达。结论 :大鼠外周血中含有EPC ,在体外特殊诱导环境下 ,可定向分化为内皮样细胞。
作者 孙龙 胡波 朱洪生 连锋 吴学军 王小妹
机构地区 上海第二医科大学附属仁济医院心胸外科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2004年第4期500-501,503,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目 (No .30 1 70 930 )
关键词 干细胞 内皮祖细胞 内皮细胞 分化
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献9

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同被引文献46

  • 1李红霞,金晓明,胡月明.内皮祖细胞的分离培养及生物学特性的鉴定[J].哈尔滨医科大学学报,2007,41(2):173-175. 被引量：1
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  • 8Li XH, Xu B. HMG-CoA reductase inhibitor regulates endothelial progenitor function through the phosphatidylinositol 3 '-Kinase/AKT signal transduetion pathway. Appl Biochem Biotechnol, 2009,157 (3) :545-553.
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细胞与分子免疫学杂志
2004年 第4期

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