基质金属蛋白酶-9和肿瘤坏死因子-α在中耳胆脂瘤中的表达

The expression of matrix metalloproteinase-9 and tumor necrosis factor-α in cholesteatoma

目的 :观察基质金属蛋白酶 (MMP) 9、肿瘤坏死因子 (TNF) α在胆脂瘤组织中的表达 ,探讨二者在胆脂瘤骨质破坏中的作用。方法 :采用免疫组织化学技术 (SP法 )和计算机图像定量分析法 ,检测TNF α、MMP 9在 2 2例胆脂瘤及 2 0例外耳道皮肤中的表达 ,同时观察二者的表达与临床上听小骨骨质破坏程度的相互关系。结果 :MMP 9表达于胆脂瘤上皮下结缔组织基质细胞 ,TNF α在上皮层及上皮下结缔组织基质细胞中有高度表达 ,定量分析显示胆脂瘤组织中二者的含量显著高于外耳道皮肤 (P <0 .0 5 ) ,并且二者的表达与骨质破坏的程度有关。结论 :MMP 9和TNF α在胆脂瘤组织中呈高表达 ,且与骨质破坏程度呈正比 ,表明其与胆脂瘤的骨质破坏密切相关 ;并且MMP 9和TNF α的表达有相关性 ,提示二者之间可能存在相互作用 ;MMP 9仅在胆脂瘤基质细胞中表达 ,TNF α也在基质细胞中表达 。

Objective:To observe the expression of MMP-9、TNF-α in cholesteatoma and to determine their roles in the destruction of bone.Method:Immunohistochemical method (SP) and the computer image quantitative analysis were used to examine the expression of TNF-α、MMP-9 in 22 samples from cholesteatoma and 20 normal cases. At the same time, the correlation between the expressions of the two indexes and the erosion of ossicles were observed.Result:MMP-9 protein was only expressed in cholesteatoma stromal cells.TNF-α was expressed in both epithlium and stromal cells.The results of the computer image quantitative analysis showed that the mean optical density of MMP-9 and TNF-α in cholesteatoma were higher than that in normal skin epithelial tissue (P< 0.05),in addition,the two indexes were relative to the extent of ossicle destruction.Conclusion:MMP-9 and TNF-α are overexpressed in cholesteatoma and they are associated with ossicle destruction,which shows that MMP-9 and TNF-α are responsible for bone destruction in cholesteatoma; MMP-9 and TNF-α have a correlation in their expression,which suggest that the two factors act on each other possibly; Overexpression of MMP-9 is only observed in stromal cells and the immunostain of TNF-α also is observed in stromal cells which indicates that stromal cells play an important role in bone destruction.