培养毛乳头细胞bFGF、ET-1、SCF的表达及其生物学特性

Expressions of bFGF,ET-1 and SCF in dermal papilla cells and the relation to their biological properties

目的 :观察不同传代培养人毛乳头细胞的 b FGF、ET- 1、SCF表达变化情况及对其诱导毛囊再生能力的影响。方法 :采用细胞培养、免疫组织化学和原位杂交技术 ,对不同传代培养的毛乳头细胞的 b FGF、ET- 1、SCF的表达变化情况进行观察 ,同时观察其对诱导毛囊再生能力的影响。结果 :低传代培养毛乳头细胞 ET- 1、SCF表达较强 ,传代 >6代后 ET- 1和 SCF转为阴性 ;并发现毛乳头细胞表达 ET- 1和 SCF越强 ,其诱导毛囊再生能力也越高。结论 :毛乳头细胞诱导毛囊再生的能力与其表达 ET- 1和

Objective: To investigate the expression of bFGF,ET-1 and SCF in different passages of cultured dermal papilla cells (DPC),and their possible effect on biological behaviour of DPC. Methods: The expression of bFGF,ET-1 and SCF in different passages of cultured DPC was detected by immunocytochemistry and in situ hybridization. Results: The expression of ET-1 and SCF in early passages of cultured DPC was stronger,but became negative in late passages (>6 passages ). The stronger the expression of ET-1 and SCF in DPC,the higher ability of DPC to induce hair follicle regeneration. Conclusions: The expression strength of ET-1 and SCF is related to the ability of DPC inducing hair follicle regeneration.