PCR在动物支原体检测中的应用

Brief Introduction of PCR and its Application in the Detection of Mycoplasma

PCR是近年来发展起来的一项分子生物学新技术 ,具有灵敏、特异、快速、简便和适用面广等优点 ,因而在分子生物学检测与研究中具有广阔的应用前景。目前 ,PCR被广泛应用于众多领域 ,其在支原体检测中的应用与发展已经取得了可喜的成绩。文章简要介绍 PCR在动物支原体检测中的应用 ,包括应用通用 PCR引物与特异性引物检测支原体 ,以及 PCR在禽类、牛和猪支原体检测中的应用现状。表明在实验室和临床中应用 PCR检测支原体是一种有效的方法。

PCR is a new molecular biology technology in recent years. Now it has wide application in realm of detection and research in molecular biology because of its strongpoints, such as sensitivity, specifici, rapidity and simplicity. This article briefly introduced its basic mechanism and the usage of PCR in animal mycoplasma that includes detections of mycoplasma by universal PCR primers and special primers. The paper also introduced its application in poultry,bovine and pig. It showd that the detection of mycoplasma with PCR would be a helpful way in laboratory and clinic.