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口蹄疫多基因杆状病毒转移载体的构建

Construction of Recombinant Baculovirus pMelBacB Transfer Vector Containing Multiple Genes of FMDV
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摘要 采用分步 PCR扩增连接的方法将口蹄疫病毒结构蛋白基因 P1 ,非结构蛋白基因 2 A、2 B、3 C蛋白酶和 3 D聚合酶按正确的读码框依次连接克隆入 p GEM-T载体。然后 ,将切取的目的基因亚克隆入杆状病毒转移载体 p Mel Bac B。构建成功的重组 p Mel Bac B/P1 2 X3 C3 D转移载体经测序鉴定 ,含有完整的目的基因表达盒 ,转移载体可与杆状病毒骨架载体进行昆虫 sf9细胞内同源重组 。 The recombinant baculovirus pMelBacB transfer vector containing FMDV type O capsid,2A,2B,3Cprotease and 3Dpolymerase coding regions were constructed by the methods of multi-step cloning and ligating PCR.The recombinant pMelBacB/P12X3C3D transfer vector were confirmed by PCR,digestion of restricted endonuclease and sequencing. The recombinant pMelBacB/P12X3C3D can be used to create recombinant baculovirus containing FMDV capsid and no-structural protein genes.
作者 云涛 冷青文 郭慧琛 刘在新 张居农 谢庆阁
机构地区 中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室 石河子大学动物科技学院
出处 《动物医学进展》 CSCD 2004年第4期104-106,共3页 Progress In Veterinary Medicine
基金 国家"973"资助项目 (G19990 1190 3)
关键词 口蹄疫 多基因杆状病毒 转移载体 基因工程疫苗 Foot-and-mouth disease virus(FMDV) baculovirus pMelBacB transfer vector
分类号 S855.3 [农业科学—临床兽医学]
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参考文献10

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动物医学进展
2004年 第4期

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