食品中副溶血性弧菌PCR快速检测方法的研究

Faster detection of Vibrio parahaemolyticus in foods by PCR technique

为建立食品中副溶血性弧菌 (VP)的PCR检测方法 ,选取tl基因作为靶序列设计一对引物 ,用该引物对 14株从国内食品中分离的副溶血性弧菌 (经传统方法验证 )和 30株非副溶血性弧菌进行PCR扩增 ,并用此方法对人工污染食品进行检测。扩增片段表现出极好的特异性 ,对人工污染的冷冻虾仁、沙丁鱼的检出限为 10CFU g ,且与传统方法结果吻合。该方法适宜于食品中副溶血性弧菌的检测。

The purpose of this study is to establish a new technique for detection Vibrio perahaemolyticus (VP) faster and validate its practicability. The new technique involves designing a primer pair targeting tl gene and using the primer pair for PCR amplification The technique was tried in 14 VP strains and 30 non-VP strains, and also in factitiously contaminated food samples. The results showed that the technique offered excellent differentiation and lower detection limit (10 CFU/g). The full course of assay could be completed in 13 hours, which was significantly shorter than the time needed by conventional techniques. It is concluded that the technique is practical with advantages of simple operation, higher specificity, less time-consuming and lower detection limit. Nevertheless, more trial is needed because the technique was validated in only 2 kinds of actual food samples.