反相液相色谱法测定白藜芦醇胶囊中的白藜芦醇

Quantitative analysis of resveraerol by HPLC

为监测保健食品中的有效成分 ,建立了高效液相色谱法测定白藜芦醇胶囊中白藜芦醇含量的方法。实验采用KromasilC18柱 ,以乙腈 +水 (体积比 30 +70 )溶液为流动相 ,用紫外检测器于30 4nm处检测。结果表明 ,白藜芦醇浓度在 10～ 2 5 0 μg/mL时 ,浓度与峰面积呈良好的线性关系 (r=0 9999) ;加标回收率为 92 5 %～ 10 2 6 % ;最低检出浓度 0 6mg/g。方法的精密度良好 ,相对标准偏差 (RSD)为 3 3%。方法快速、简便、准确 ,可作为胶囊类保健食品质量检验的一个定量检验方法。

Resveraerol in resveraerol capsule was quantitatively determined by high performance liquid chromatography with Kromasil C 18 column,a mixture of CH -3CNH+H -2O(30+70,volume ratio)as mobile phase and UV detection at 304 nm.There was a good linear relationship wtihin the concentration of 10～250 μg/mL( r 0.9999).The recovery was 92 ^5%～102 ^6% and the relative standard deviation was 3 ^3%( n 6 ).The detection limit was 0.6 mg/g.The method is rapid,simple,accurate and reproducible.The method is proposed to be used for quality control of aloe products.