基于Michaelis-Menten型呼吸速率模型的实验研究

Experimental Study on Michaelis-Menten Type Respiration Model

冷藏结合气调包装是果蔬采后保持品质的有效手段 ,呼吸速率是系统设计的必要依据 .以黄桃为例 ,确定了 0、5、1 0、1 5和 2 5°C下呼吸速率随 O2 、CO2 浓度的变化规律 ,建立了基于酶动力学原理的 Michaelis- Menten型黄桃呼吸速率模型 .结果显示 ,呼吸速率随 CO2 浓度的增加而减小 ,最大呼吸速率随温度的增加而增加 ,并满足 Arrhenius关系 .该方法适用于包括非平衡状态在内的任何阶段 .

Refrigeration and modified-atmosphere packaging (MAP) are effective methods in quality control for postharvest fruits and vegetables. The respiration rates are essential to the design of MAP system. A respiration model, based on Michaelis-Menten type enzyme kinetics, was proposed for predicting respiration rates of yellow peaches as a function of O_2 and CO_2 concentrations at the storage temperature of (0 °C,) 5 °C, 10 °C, 15 °C and 25 °C. It was shown that the respiration rates decrease with increasing CO_2 and increase with increasing temperature. The temperature dependence of the maximum respiration rates follows the Arrhenius relationship. This method can be used in the whole storage period including steady or unsteady state. The respiratory heats calculated from the respiration rates can be used to determine the cooling load in cold storage.