高效氯氰菊酯对德国小蠊乙酰胆碱酯酶抑制效应研究

Inhibition of α-cypermethrin on Acetylcholinesterase of Blattella germanica

目的 通过研究高效氯氰菊酯与德国小蠊乙酰胆碱酯酶 (AChE)活性水平的量效与时效关系 ,进一步完善高效氯氰菊酯作用机制的研究 ,为德国小蠊的防制工作提供理论基础和参考方法。方法 参照国标GB13 917.1～GB13 917.8-92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氰菊酯 ,用乙酰胆碱酯酶试剂盒测定德国小蠊AChE活性。结果 48h内高效氯氰菊酯对德国小蠊AChE活性的抑制作用呈浓度梯度效应 ,高效氯氰菊酯的浓度与德国小蠊 72h的死亡率和 14 4h内对AChE活性的抑制时效呈正相关。结论 德国小蠊的乙酰胆碱酯酶是高效氯氰菊酯的作用靶标之一 ,由于AChE敏感度降低而产生的靶标抗性是德国小蠊对高效氯氰菊酯产生抗药性的原因之一 ,德国小蠊AChE活性水平可做为对拟除虫菊酯类杀虫剂抗性水平检测的指标之一。

Objective To more perfectly understand the operational mechanisms of α-cypermethrin and provide theoretical basis and useful methods by researching dosage effect and time effect between α-cypermethrin and acetylcholinesterase(AChE) activities in Blattella germanica . Methods Quantitative α-cypermethrin at different rate was sprayed based on the method of GB13917.1- GB13917.8-92 for insecticide registered testing indoor effect. The activities of AChE were determined by using Acetylcholinesterase(AChE) Kit. Results Inhibition of α-cypermethrin on AChE activities of Blattella germanica showed concentration gradient effect in 48 hours. The results showed the correlation of concentration of α-cypermethrin and inhibiting time effect on AChE activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship. Conclusion AChE is one target of α-cypermethrin in Blattella germanica . Because of sensitivity decrease of AChE,target resistance is one of the important resistant mechanisms to α-cypermethrin. The activity of AChE in Blattella germanica may be one of the indices for determining resistant level to pyrethroid insecticides.