以可控孔径玻璃为基质的疏水作用固定相的研制

Development of the Stationary Phases Based on Controlled Pore Glass Used for High-performance Hydrophobic Interaction Chromatography

以不同分子量的聚乙二醇(PEG 400,600,800,1000和2000)为配基,发展了一类以可控孔径玻璃(CPG)为基质的疏水作用色谱固定相,研究了其分离生物高分子的色谱条件,这类固定相可以以很高的活性回收率使蛋白质得到满意的分离。

A series of hydrophobic interaction chromatographic stationary phases based on controlled pore glass have been developed. The ligand was polyethylene glycol(PEG) with different degree of polymerization, such as PEG 400, 600,800,1000, and 2000. Chromatographic conditions used for the separation of biopolymer were evaluated. This packing is suitable for the separation of protein with high recovery of bioac-tivity.