过氧化氢歧化酶模拟酶的合成及催化性质的研究——meso-四(4-磺基苯)卟啉-锰(MnTPPS_4)作为模拟酶的研究

Synthesis of Mimetic Catalase and Its Catalytic Activity——Manganese Porphyrin Acts as Mimetic Enzyme

锰和meso-四(4-磺基苯)卟啉的螯合物MnTPPS_4可以作为过氧化氢歧化酶的模拟酶催化H_2O_2分解。本文首次探索将该模拟酶应用于生物传感器方面的可行性。在25℃、pH8.0条件下,模拟酶电极对1.0×10^(-3)～7.0×10^(-3)mol/L浓度范围的H_2O_2的响应呈线性。相关系数为0.9964。该电极的重现性,回收率良好,具有一定的抗干扰能力。这为进一步将该模拟酶代替天然酶应用于生物传感器奠定基础。

A decomposition reaction of hydrogen peroxide by mimetic enzyme of catalase [manganese-te-trakis (sulfophenyl)porphyrin] was investigated. The possible application of the mimetic enzyme has been confirmed in the successful construction of a biosensor. The linear response of mimetic enzyme electrode is over the range of 1.0×10-3-7.0×10-3mol/L for hydrogen peroxide. The electrode shows good reproducibilityi recovery and a proper ability of anti-interference.