组氨酸-铜体系吸附伏安法的研究——血清、果汁中组氨酸的测定

Adsorptive Voltammetry of the Histidine-Copper System——Determination of Histidine in Serum and Natural Fruit Juice

在pH9.2的硼砂溶液中,组氮酸-铜络合物在-0.36V产生还原峰峰电流与组氨酸浓度在5.0×10^(-9)～1.0×10^(-7)mol/L.内呈线性关系,检测下限为5.0×10^(-10)mol/L。本法可用于血清及天然果汁中组氨酸的测定。

The behaviour of the histidine complex with copper (Ⅱ) at a hanging mercury electrode has been investigated in borate buffer solution(pH9. 2) and the mechanism is discussed. The reduction peak potential of the histidine-copper(Ⅱ) complex is-0. 36 V(vs. SCE). The linear relationship between the concentration of histidine and the peak current is in the range of 5.0×10-9-1. 0×10-7mol/L,the detection limit is 5. 0×10-10 mol/L. This method has been used for the direct determination of histidine in serum and natural fruit juice with satisfactory results.