摘要
2001年在湖南省安化,桃江和松柏3县稻瘟病圃内采集稻瘟病标样并分离鉴别其交配型和中国稻瘟病菌的致病型.用经过筛选的13对RAPD引物分析了其中44个菌株的遗传多样性,采用UPGMA法聚类,用DPS 2000软件进行分析,将测试菌株区分为6个不同的遗传宗谱,在遗传距离＜8.42处的遗传宗谱Ⅳ和Ⅴ的优势群体中,分别包含11个菌株,而归属于宗谱Ⅲ的仅包含有1个具有特殊遗传背景的菌株.交配型1.1和交配型1.2的中国不同致病型菌株可归属为同一宗谱,在同一遗传宗谱内又可存在不同的生理小种.研究结果证实了湖南省病圃稻瘟病菌的遗传多样性和致病复杂性.
Diseased samples of Magnaporthe grisea were collected from Anhua, Taojiang, Songbai blast nurseries in Hunan province in 2001. In the previous study the each isolate was characterized by the mating type and Chinese pathotypes. Forty-four isolates tested were analyzed with 13 selected random amplified polymorphic DNA (RAPD) primers in the present work. The isolates tested were classified into 6 different genetic groups (lineages) by UPGMA using the software DPS 2000. Among them lineages Ⅳ and Ⅴ were the predominant one each with 11 isolates while lineage Ⅲ clustered only one isolate. The isolates with Mat 1.1 and Mat 1.2 belonging to different Chinese pathotypes were grouped in the same lineage; moreover isolates of each genetic lineage belonged to different Chinese physiological race. The results obtained confirm the genetic diversity and virulence complexity of rice blast fungus in Hunan province.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第4期355-362,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金
