摘要
A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.
A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第8期1451-1453,M010,共4页
Chemical Journal of Chinese Universities
基金
国家重点基础研究发展计划项目(批准号 :0 0 1CB5 10 2 0 2 )资助
关键词
微透析中空纤维膜接口
二维分离平台
毛细管等电聚焦
毛细管区带电泳
Microialysis hollow fiber membrane interface
Two-dimensional separation platform
Capillary isoelectric focusing
Capillary zone electrophoresis
