摘要
建立了一种利用微量荧光计定量测定双链DNA及PCR扩增产物的简便方法。在dsDNA含量为5～250ng的范围内,荧光强度与dsDNA含量呈线性关系。用此法测定PCR产物量时,荧光信号不受石蜡油或反应混合物中剩余产物及4×dNTP的干扰,因为这些成份均非双键DNA。本法试用于临床检验,定量测定乙型肝炎病毒DNA及PCR扩增产物,测定结果与用目测电泳胶中溴乙锭带亮度的半定量法符合良好。
A simple method for the quantitative determination of double stranded DNA and PCR products by microfluorometer is described. There is a linear relationship between the intensity of fluorescence and the content of dsDNA within the range of 5～250ng DNA/mL,Other UV absorbing materials with single strand present in the reaction mixture do not interfere with the determination.
出处
《分析仪器》
CAS
1993年第3期48-52,56,共6页
Analytical Instrumentation
