摘要
In the condition of pH 2.5～4.7,ethyl eosin reacts with sildenafil to form red ion association complexes.The maximum absorption wavelengths and the maximum fading wavelengths were 550 nm and 520 nm,respectively.The apparent molar absorptivities（ε）were 2.09×104 and 2.44×104L·mol-1·cm-1,respectively.When the dual-wavelength overlapping absorption spectrometry was used to determine the sidenafi,their apparent molar absorptivity ε was increased to 4.57×104 L·mol-1·cm-1.The method was applied to the determination of the content of the agora sell and in human urine sildenafils with satisfactory results.
In the condition of pH 2.5～4.7,ethyl eosin reacts with sildenafil to form red ion association complexes.The maximum absorption wavelengths and the maximum fading wavelengths were 550 nm and 520 nm,respectively.The apparent molar absorptivities(ε)were 2.09×10~4 and 2.44×10~4L·mol^(-1)·cm^(-1),respectively.When the dual-wavelength overlapping absorption spectrometry was used to determine the sidenafi,their apparent molar absorptivity ε was increased to 4.57×10~4 L·mol^(-1)·cm^(-1).The method was applied to the determination of the content of the agora sell and in human urine sildenafils with satisfactory results.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2004年第4期573-574,共2页
Chemical Research and Application
关键词
光度法
枸橼酸西地那非
尿样
乙基曙红
spectrophotometry
sildenafil citrate
urine sample
ethyl eosin
