摘要
采用硝酸铝———亚硝酸钠比色法对迎春花茎叶内的黄酮类物质含量进行了初步测定 ,确定了总黄酮含量提取的适宜溶剂浓度和提取的适宜时间。结果表明 ,用 6 0 %甲醇溶剂提取 5h总黄酮含量最高 ,茎杆中的含量为 10 4 2mg gDW ,叶片中的含量为 18 31mg gDW。此外 ,还对迎春花叶提取液进行了各种黄酮定性颜色反应 。
The oven-dried leaves and stems from Jasminum nudi-florum Lindl.were analyzed for the total contents of flavonoids by spectrophotometryic method of aluminum nitrate-natrium nitrite.And the optimal solvent concentration and the best time of extraction were also obtained.Results showed that using 60% Methyl alcohol for 5 hours of extraction could achieved the highest contents of total flavonoids.The total contents of flavonoidsare 10.42(mg/g DW) in stems,18.31(mg/gDW) in leaves.In additional,the total flavoids extraction from Jasminum nudiflorum Lindl.are mainly Flavanones and Chalcones by analyzing for its coloration reactions.
出处
《生物技术》
CAS
CSCD
2004年第4期30-31,共2页
Biotechnology
关键词
塑春花
总黄酮
含量
颜色反应
Jasminum nudiflorum Lindl.
total flavonoids
contents
coloration reactions
