6-二甲基氨基嘌呤诱导扇贝异源三倍体

Allotriploid scallop induction by 6-Dimethylaminopurine

用 6 二甲基氨基嘌呤处理栉孔扇贝♀×虾夷扇贝♂受精卵 ,抑制第二极体释放诱导异源三倍体。水温 17℃下 ,分别进行了不同起始处理时间 (受精后 2 0～ 4 0min)、不同药物处理浓度 (4 0～ 80mg/L)和不同持续处理时间 (5～ 2 0min)的实验。起始处理时间实验以受精后 35min开始处理效果最好 ,三倍体率为 5 1 8% ,D形幼虫成活率可达 78% ;药物处理浓度和持续处理时间最佳组合为 6 0～ 70mg/L 6 DMAP处理 2 0min ,三倍体率可达 5 9 6 5 % ,D形幼虫成活率为 2 2 0 5 %。综合考虑 ,诱导扇贝异源三倍体的适宜参数为 :当 5 0 %受精卵排出第一极体时 ,以 6 0～ 70mg/L 6 DMAP处理 2 0min。

Fertilized eggs crossing between Chlamys farreri(♀) and Patinopecten yesoensis(♂) were treated with 6-dimethylaminopurine to induce allotriploid by inhibiting the release of second polar body. Different treatments of 6-DMAP, including initial treating time( 20～40 min after fertilization), concentration(40～80 mg/L) and duration(5～20 min), were tested at 17 ℃ water temperature. The optimal initial time was 35 min after fertilization, which resulted in 51.8% allotriploid percentage and 78% survival rate of D-larvae. The best combination of concentration and duration was 60～70 mg/L 6-DMAP with 20 mins treatment. The allotriploid percentage and survival rate of D-larvae were 59.65% and 22.05% respectively. Overall, the most effective procedure for inducing allotriploid of scallop Chlamys farreri(♀) ×Patinopecten yesoensis(♂)was to treat fertilized eggs for 20 mins by 60～70 mg/L 6-DMAP when 50% fertilized eggs released the first polar body.