黄山松群体GOT同工酶遗传变异的初步研究

Preliminary Study on Genetic Variation of GOT Isozyme in Taiwan Pine Populations

利用黄山松(Pinus taiwanensis Hayata)4个天然群体和种源试验林中12个种源种子胚乳,分析了GOT同工酶。结果表明:GOT同工酶共有4～6条谱带,分成5个染色区受5个位点控制,所分析的1～4个位点均是多态的,共有12个等位基因,依据12个等位基因频率所估算的种源间遗传距离为0.0038～0.3313,且遗传距离同相应种源间的地理距离无直线关系。经相关分析知基因频率中既有地理倾变的,且与降水量、日照时数等气象因子有关,也有随机变异的。用遗传距离对参试种源进行聚类只能区分出大别山类群。

GOT isozyme was analysed through endosperm from 4 natural populations and 12 provenance seeds of Taiwan pine provenance test plantation, in Zhejiang province. The results showed that there are 4-6 bands on the GOT isozyme of these endosperms; that the bands can be divided into 5 dyeing regions with each region controlled by a site; that the first-fourth sites analysed were of all morphism; that there were 12 alleles in the 4 sites; that the genetic distances estimated, based on the gene rates of 12 alleles , were 0.0038-0.3313 between provenances; and that there is no linear relation between the genetic distance and the geographic distance of provenances. And multistatistic analysis showed that some gene rates have a clinal pattern with geographic factors, which is related to annual rainfall and annual sunshine hours $ and that the other gene rates variated randomly. Only Dabieshang group could be isolated from 12 provenances on the genetic distance by cluster analysis.