穞稻分类地位的SSR证据

SSR Evidence for Taxonomic Position of Weedy Rice‘Ludao’

穞稻是发现于我国江苏连云港地区(北纬34°33′～34°46′)具有杂草生长习性的一类稻种资源。本研究采用30对水稻SSR引物;;比较穞稻与22份近缘野生种、21份亚洲栽培稻种质间的遗传差异。结果表明;;穞稻有14对SSR引物的带型与源于安徽省的4份塘稻材料相同;;存在10个与粳稻和野生稻共同的SSR位点等位基因;;并携带2个籼稻特征标记位点等位基因和1个普通野生稻特征位点(RM25)等位基因。在RM82位点上;;发现1个穞稻、塘稻、深水稻和普通野生稻材料所共有的等位基因。聚类分析和主成分分析则显示穞稻与安徽塘稻遗传距离最近;;与太湖栽培粳稻明显不同。认为穞稻是保留较多普通野生稻等位基因的较原始亚洲栽培稻种粳稻类型。

Ludao is a type of weedy rice grown naturally in field of Lianyungang region (34°3334°46N), JiangsuProvince, China. Thirty SSR primers in rice were used for the study on the genetic variation in Ludao, cultivated and wildrice. The results showed that Ludao shared common bands in 14 SSR markers with 4 accessions of the type of deep-waterrice originated from Anhui Province, showed 10 japonica-specific and 2 indica-specific SSR loci, and had a common wildrice-specific allele at RM25 locus. One locus of RM82 was found only in Ludao, deep-water rice, and common wild rice (O.rufipogon Griff). Both UPGMA clustering and principal component analysis indicated Ludao was most close to the type ofdeep-water rice from Anhui Province, and quite distinct from Taihu japonica rice. It is concluded that Ludao may be aprimitive japonica rice of O.sativa L., which remained more allele loci of common wild rice (O.rufipogon Griff).