核盘菌重寄生菌链孢粘帚霉HL-1-1菌株的生物学特性研究

Biological characteristics of Gliocladium catenulatum HL-1-1 mycoparasited on Sclerotinia sclerotiorum

对植物真菌病害优良生防菌株链孢粘帚霉 (Gliocladiumcatenulatum)HL 1 1的生物学特性进行了研究。结果表明 ,以核盘菌菌核粉为唯一碳源的培养基最适合HL 1 1的菌丝生长 ,PDA最适合产孢 ;蔗糖作为碳源、硝酸钾作为氮源、2 5 30℃、pH值 5及黑暗等条件适合HL 1 1的菌丝生长 ;可溶性淀粉作为碳源、牛肉膏作为氮源、2 5℃、pH值 6及光照等条件适合产孢 ;菌核浸出液对HL 1 1的分生孢子萌发有促进作用 ,葡萄糖对分生孢子萌发有抑制作用 ;2 5 30℃及黑暗条件适合孢子萌发 ,温度低于 5℃或高于 4 0℃ ,孢子不能萌发 ;孢子致死温度为 5

Biological characteristics of G. catenulatum HL-1-1, a superior isolate for biological control of plant fungal diseases were studied. The results showed that medium made from powder of fungal sclerotia was optimum for the growth of this isolate, while PDA was optimum for its sporulation. Sucrose was optimum C source and KNO_3 optimum N source for its growth. Soluble starch was optimum C source and beef extract optimum N source for its sporulation. Temperature ranged from 25 to 30℃ was optimum for its hyphae growth and spore germination, while 25℃ was optimum for sporulation. Spores could not germinate below 5℃ or over 40℃. Lethal temperature of spores was 52℃. Optimum pH was 5.0 for hyphae growth and 6.0 for sporulation. Dark condition enhanced hyphae growth and light was good for sporulation. Extract of sclerotia promoted spore germination of the isolate,but D-glucose inhi-(bited.) The data provide basic information for further studies on fermentation, formulation and delivery techniques of the isolate.