拟南芥抗盐突变体的RAPD分析

A RAPD analysis on salt-tolerance mutant of Arabidopsis thaliana

以筛选得到可以稳定遗传的抗盐单基因突变体2#和15#以及野生型拟南芥为材料进行RAPD分析,150条引物中有3条引物在突变体之间扩增出多态性,不仅证明了DNA水平突变的发生,而且表明它们之间遗传背景相似,是一系列抗盐性不同的近似等位基因系.1条引物在突变体的扩增产物比在野生型的扩增产物多出一个大小约为1200bp的片段,初步认为该片段与抗盐的主效基因有关.

Wild Arabidopsis thaliana and two salt-tolerance mutants were used for RAPD analysis.Three out of 150 radom primers could detect DNA polymorphism between the two mutants and the result showed that they were a series of near isogenic lines because of their little difference and similar genetic back ground.Only one primer gave a specific DNA fragment about 1 200 bp which two mutants own but the parent don't.We drew a preliminary conclusion that the fragment is releted to the major salt-tolerance gene.