用于克隆的猕猴耳成纤维细胞的培养及其有关生物学特性

Culture and Related Characteristics of Macaque Ear Fibroblasts Used for Cloning

应用组织块培养法成功地分离培养了猕猴耳皮肤成纤维细胞。对培养细胞进行了形态观察、生长曲线测定、免疫细胞化学分析、染色体和周期分析,结果表明培养的细胞具有正常的大小、形态、细胞骨架系统和染色体数目,而且随着细胞汇合程度的增加,G0／G1期细胞所占的比例上升,70％-80％和90％-100％两种汇合程度的G0/G1期和S期比例间存在明显的差异(P<0.05)。对培养猕猴耳成纤维细胞的有关生物学特性进行分析,有利于给同种和异种体细胞克隆猴研究提供形态良好、染色体数目正常的供体细胞,同时也为体细胞转基因等其他领域的研究提供了基础条件。

Fibroblasts derived from macaque (Macaca mulatta tcheliensis) ear were successfully cultured with a normal tissue culture method. The cultured cells were analyzed by their morphology, skeleton, chromosomes and cell cycle. Results demonstrated that these cultured cells were typical flbroblasts with normal morphology, cell skeleton and karotype, and G0/G1 rate increased with the confluence of cells. Marked difference existed in the G0/G1 and S stage rate of 70%-80% and 90%-100% confluence. The culture and characterization analysis of macaque flbroblasts provided not only a ready supply of somatic cells for the intraspecies and interspecies cloning of macaque but also fundamental conditions for somatic transgene and other related fields.