615小鼠胚胎移植初探

Study on Embryo Transfer in 615 Mice

目的 提高 6 15小鼠胚胎移植成功率。方法 采用经超量排卵的 6 15雌鼠与正常雄鼠交配 ,取受精卵 ,再经手术分别将单胚卵、双胚卵移入发情的假孕雌鼠的输卵管中 ,妊娠产仔。结果 单胚卵移卵后妊娠率为6 3 3%、产仔率为 35 8% ;双胚卵移入第 1 5天、第 0 5天的假孕雌鼠妊娠率为 0、72 % ,后者的产仔率为 5 2 7% ;结论 移植双胚卵可提高 6

Objective To improve the success rate of embryo transfer in 615 mice. Methods\ Each superovulated female of 615 mice was placed individually with a stud male and was checked the next morning. The fertilized eggs were collected from the oviducts of the plugged females. Embryos from the one cell stage to the two cell stage were transferred to the ampullae of pseudopregnant recipients in estruation to complete their development. Results\ By transferring embryos from the one cell stage, the rate of pregnancy was 63 3%,19% of embryos developed to term; by transferring embryos from the two cell stage to 1 5 and 0 5 day pseudopregnant recipients, the rates of pregnancy were 0,72% separately. 34 7% of embryos transferred to 0 5 day pseudopregnant developed to term. Conclusion\ More embryos from the two cell stage develop to term than those from the one cell stage.