对环磷酰胺引起低白细胞大鼠的白细胞分类变化研究

Study on the Change of Leucocyte Classification in Leucocyte Decreasing Rats Induced by Cyclophosphamide

目的 研究环磷酰胺引起低白细胞大鼠的白细胞分类变化。方法 给大鼠腹腔注射环磷酰胺(30mg kg) ,连续 5天 ;对照组注射等体积的生理盐水。结果 大鼠外周血白细胞总数明显减少且低水平维持时间较长 ;杆状核粒细胞明显增高 ,停药 15天 ,仍维持在较高水平 ;分叶核粒细胞在给药期间有明显减少 ,停药后即恢复 ;对淋巴细胞没有影响。结论 给大鼠反复注射环磷酰胺能引起白细胞总数及其分类的变化 ,杆状核粒细胞显著升高和分叶核粒细胞明显降低 。

Objective To observe the change of leucocyte classification in leucocyte decreasing rats induced by cyclophosphamide.Methods\ Cyclophosphamide (30/kg) was injected into rats' abdominal cavity for five days,while in the control group,isovolumic normal saline was injected.Results\ The total of leucocyte in peripheral blood decreased markedly( p <0 01),which maintained for 15 days after stopping dosing.In the mean time,band\|form granulocyte increased (p<0.01),even if stopping dosing 15 days;segmented granulocyte reduced (p<0.01),but it returned after stopping dosing.Cyclophosphamide had no effect on lymphocyte.Conclusion\ Cyclophosphamide caused the change of leucocyte count and its classification, with band\|form granulocyte increased and segmented granulocyte reduced,which could be used as experimental indications.