神经细胞尼氏体和神经髓鞘的双重组合染色法与应用

Application for a Double-compound Staining Method Showing Nissl Bodies and Medullary Sheath

探讨了显示实验动物脊髓组织中的神经尼氏体和神经髓鞘等组织成分的双重组合染色法 ,通过分别选用孔雀石绿 (Malachitegreen)和橙黄G、磷钨酸 (OrangeG ,Phosphotungsticacid )组合染色 (简称MG OP法 )。已能够显示狗脊髓神经尼氏体呈绿色 ,细胞核呈黄色。神经纤维髓鞘轴突呈黄色 ,神经膜和结缔组织纤维呈绿色 ,背景呈淡黄色。MG OP法克服了原法成分单一 ,色彩效果差 ,所建立的双重组合染色法 ,对比清晰 ,色彩鲜艳 。

To develope a method for staining Nissl bodies and medullary sheath in dog spinal cord tissues, the double-compound staining dyes composed of Malachite green, Orange G and Phosphotungstic acid (MG\|OP) were used. The Nissl bodies,nerve membrane and connective tissue were stained green, the medullary sheath and cell nuclei were stained yellow, and the background showed light yellow. MG\|OP double\|compound staining method possess more vivid color and more striking contrast than the original method, so it is an ideal compound staining method for morphological study.