摘要
本文对旨在以脱毒为目的的甘蔗茎尖培养技术进行了探讨,茎尖长约2mm,成功的关键在于防止酚的污染,采用液体培养,并在培养过程中将培养物转移到新鲜的培养基中,能成功地防止酚的污染,并使茎尖成苗率达50％以上。促根率达80％以上,结果还表明,细胞分裂素是茎尖成苗所必需的激素,而萘乙酸则不是;侧茎茎尖比主茎茎尖易成苗;适当提高蔗糖的浓度及在培养基中加入活性炭均有利于茎尖苗发根,文中还对防止酚污的机理作了分析。
This paper reported the selection for the technique of sugarcane apical meristem culture which was aimed at producing virus-free plants. The key to success was about how to prevent phenol pollution. We used 2 mm apical meristems and succeeded in it by mcans of liquid culture and constantly translating materials to fresh mcdinm. Thr ratrs of plantlrt and root formation wrrr ovrr 50% and 80% respectively. Result also showed that BA was a ncccssary auxin, but NAA not; Lateral stalk was better for plantlet formation than main stalk; Increasing sucrosc concentration and adding active carbon in medinm were of benefit to the formation of roots. Bcsides, we also analyscd the mechanism of preventing phenol pollution.
基金
国家和福建省自然科学基金
关键词
茎尖培养
育种
sugarcane
apical meristem culture technique
selection
