期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰技术在几项研究领域的应用 被引量：2

Application of RNA Interference to Several Research Fields
下载PDF
导出
摘要 作为一项新的反向遗传学技术 ,RNA干扰技术正在越来越多地应用于包括鉴定基因功能、疾病治疗、植物病毒抗性研究在内的多项研究领域。在鉴定基因功能研究中 ,由于该技术的操作简便性 ,使得在基因组水平进行大范围的基因功能鉴定成为可能 ;而针对致病相关基因、致病病毒基因组进行RNA干扰 ,可以有效抑制病情恶化 ,有可能成为未来疾病治疗的重要手段 ;同时 ,将RNA干扰技术应用于植物抗病毒研究 ,为工程化植物抗病毒遗传育种提供了一个高效、特异的抗性获得手段。对RNA干扰技术在上述三个研究领域的应用作简要综述 。 As a new reverse genetic technology,RNAi is used widely in kinds of research fields,including detection of gene function,diseases therapy,plant resistance to virus and so on.Basing on the simplicity of this technology,it makes possible to detect numbers of genes in a large scale as genome level.Targeting the genes related to diseases and genome of viruses,RNAi is able to suppress diseases exacerbation,and is potential to be used in molecular therapy.Furthermore,RNAi provides an available tool to endow plant with virus resistance.This paper reviewed the application of RNAi to the three fields above and discussed several problems that should be noticed in the application of RNAi.
作者 燕飞 成卓敏
机构地区 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
出处 《中国生物工程杂志》 CAS CSCD 2004年第8期14-19,共6页 China Biotechnology
关键词 RNA干扰 双链RNA 同源序列 RNAI 反向遗传学技术 基因 疾病 治疗 病毒 RNA interference Double stranded RNA Homologous sequence
分类号 Q522 [生物学—生物化学]
  • 相关文献

参考文献31

  • 1Boutros M, Kiger AA, Armknecht S, et al. Genome-Wide RNAi analysis of growth and viability in Drosophila cells [ J ]. Science,2004,303(5659): 832 - 835
  • 2Kim YO, Park SJ, Balaban RS, et al. A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryos [ J ] . Proc Natl Acad Sci U S A, 2004,101 ( 1 ):159 - 164
  • 3Ashrafi K, Chang FY, Watts JL, et al. Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes [ J ]. Nature, 2003,421(6920): 268 - 272
  • 4Pothof J, van Haaften G, Thijssen K, et al. Identification of genes that protect the C. elegans genome against mutations by genomewide RNAi[ J]. Genes Dev,2003,17(4): 443 - 448
  • 5Berns K, Hijmans EM, Mullenders J, et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway[ J ]. Nature, 2004,428 (6981 ): 431 - 437
  • 6Paddison PJ, Silva JM, Conklin DS, et al. A resource for large-scale RNA-interference-based screens in mammals [ J ]. Nature, 2004,428(6981): 427 - 431
  • 7Song E, Lee SK, Wang J, et al. RNA interference targeting Fas protects mice from fulminant hepatitis [ J ]. Nat Med, 2003,9 ( 3 ):347 - 351
  • 8Yang G, Cai KQ, Thompson-Lanza JA, et al. Inhibition of breast and ovarian tumor growth through multiple signaling pathways by using retrovirus-mediated siRNA silencing against Her-2/neu gene expression[J] .J Biol Chem,2004,279(6): 4339 ～ 4345
  • 9Lima RT, Martins LM, Guimaraes JE, et al. Specific dowaregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells[J].Cancer Gene Ther,2004,11(5): 309 ～ 316
  • 10Hemarm MT, Fridman JS,Zilfou JT,et al. An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo [ J ]. Nat Genet, 2003,33 (3): 396 - 400

同被引文献19

  • 1燕飞,成卓敏.病毒编码的转录后沉默抑制蛋白[J].生命的化学,2004,24(4):281-284. 被引量：4
  • 2宋晓宇,孙鲁宁,张海鹏.基因治疗的新武器——RNA干扰技术的应用现状[J].国外医学（生理病理科学与临床分册）,2004,24(4):379-381. 被引量：2
  • 3燕飞,成卓敏.RNA干扰机制研究进展[J].遗传,2005,27(1):167-172. 被引量：7
  • 4Meister G,Tuschl T.Mechanisms of gene silencing by double-stranded RNA.Nature,2004,431(7006):343-349.
  • 5Qu F, Morris TJ. Suppressors of RNA silencing encoded by plant viruses and their role in viral infections. FEBS Lett, 2005, 579(26): 5958--5964.
  • 6Wang MB, Abbott DC, Waterhouse PM. A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to Barley yellow dwarf virus. Mol Plant Pathol,2000, 1(6): 347--356.
  • 7Tenllado F, Diaz-Ruiz JR. Double-stranded RNA-mediated interference with plant virus infection. J Virol, 2001,75(24): 12288-- 12297.
  • 8Kalantidis K, Psaradakis S, Tabler M, Tsagris M. The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus. Mol Plant Microbe Interact, 2002, 15(8): 826--833.
  • 9Wang MB, Metzlaff M. RNA silencing and antiviral defense in plants. Curr Opin Plant Biol, 2005, 8(2): 216--222.
  • 10Cheng ZM, He XY, Chen CC, Zhang J, Xiao H, Zhou GH.Barley yellow dwarf virus coat protein gene and transgenic wheat plants obtained by pollen tube path way. Prog Nat Sci, 1994, 4(2): 235--240.

引证文献2

二级引证文献6

中国生物工程杂志
2004年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部