广东地区幽门螺杆菌空泡毒素基因亚型及其与胃肠疾病的关系

vacA gene subtypes of Helicobacter pylori in GuangDong district and its relationship with gastrointestinal diseases

目的:1)调查广东地区患者感染幽门螺杆菌(Hp)的空泡毒素基因(vacA)亚型的流行情况;2)探讨不同的 vacA 亚型与 Hp 相关胃肠疾病间的关系。方法:自广东地区不同疾病患者胃黏膜中分离得到191株 Hp,抽提各株菌的总 DNA,采用特定引物对各株菌 vacA 基因中间序列(m)及信号序列(s)进行 PCR 检测。结果:1)广东地区患者感染 Hp vacA 基因亚型有 sla/m2、sla/mlb、slb/m2、slb/mlb、s2/m2及 sla/mlb-m2六种组合,各亚型所占比例分别为88.0%(168/191)、7.3%(14/191)、3.1%(6/191)、0.5%(1/191)、0.5%(1/191)及0.5%(1/191);2)vacA 亚型在不同 Hp 相关胃肠疾病中的检出率无显著性差异。结论:1)广东地区 Hp vacA 基因亚型以 sla/m2占绝大部份;2)不能单纯以vacA 亚型作为预测感染 Hp 后的疾病转归。

Objective 1)To investigate the epidemiology of vacA gene subtype of Helicobacter pylori (Hp) in GuangDong district.2)To assess the relationship between vacA subtypes and Hp related gastroin- testinal diseases.Methods 191 Hp strains were isolated from patients with different gastric and duodenal diseases in GuangDong district.Bacterial DNA from all these strains were extracted,and the vacA alleles were typed using PCR with specific primers.Result 1)There were six vacA mosaicisms.168 for s1a/m2 (88.0%),14 for s1a/m1b(7.3%),6 for s1b/m2(3.1%),1 for s1b/m1b(0.5%),s2/m2(0.5) and s1a/ m1b-m2 (0.5%) respectively.2)vacA subtypes have not obvious difference in various Hp associated gas- trointestinal diseases.Conclusion 1) Hp avcA s1a/m2 subtype is predominant in GuangDong district.2) va- cA subtypes couldn't be used as a predictor for diseases outcomes simply.