摘要
对藏红花(Crocus sativus L.) 愈伤组织的诱导条件进行了优化。结果表明:MS 是藏红花芽愈伤组织的最佳诱导培养基,而 B5是 叶子和花愈伤组织的最佳培养基。藏红花芽、叶和花愈伤组织的最佳诱导温度分别是18℃、25℃和21℃。光照是叶子愈伤组织诱导的有利因素,但不利于芽和花愈伤组织的诱导。1.5～2.0 mg.L-1 NAA和 0.25 mg.L-1 6-BA是愈伤组织诱导的最佳激素组合。通过目视法和HPLC方法, 从229株细胞系中筛选出细胞系Corm1,其藏红花素1 的含量是1 677 mg.g-1,生长较快,且不易褐化。为采用植物细胞工程法解决藏红花素1资源短缺问题打下了基础。
Induction conditions of Crocus sativus L. callus were optimized. MS medium was optimalfor shoot callus induction while B5 medium was optimal for leaf and flower callus induction. 18℃, 25℃ and 21℃ were the optimal temperature for callus induction of shoot, leaf and flower, respectively.Light was positive factor for leaf callus induction but negative for shoot and flower induction.Naphthaleneacetic acid (NAA, 1.5-2.0 mg.L-1) and 6-benzyladenine (6-BA, 0.25 mg.L-1) were theoptimal hormones for callus induction. Under the optimized conditions, the induction rate reached60% for shoot callus, 80% for leaf callus, and 35% for flower callus. Using eyesight and HPLCmethods, one callus line which gave rise to 1 677 mg.g-1 crocin and would not brown was obtainedfrom 229 callus lines.
出处
《植物学通报》
CSCD
北大核心
2004年第4期455-460,共6页
Chinese Bulletin of Botany
基金
Foundation item: 863 Program.
