不同生长调节物质对苎麻愈伤组织诱导的影响

Effects of Different Growth Regulators on Ramie Callus Induction

用3种细胞分裂素和4种生长素类生长调节物质进行苎麻(Boehmerianivea)单因素、双因素诱导愈伤组织试验,结果表明苎麻的脱分化容易。在培养基中仅加2,4 D和6 BA均能较快地启动苎麻下胚轴、子叶、叶、茎外植体的脱分化。6 BA与2,4 D、IBA、IAA、NAA组合使用诱导愈伤效果均较好,生长势强;ZT和IBA、2,4 D组合、KT与2,4 D和NAA组合均有好的诱导效果。细胞分裂素类用量范围2 0～2 5mg/L,生长素类用量范围0 2～1 0mg/L,对诱导苎麻愈伤发生及增殖有较好的效应。

Three cytokinins(CTK) and four auxins(AUX) were used as growth regulators in single-factor and double-factor test. The results showed that callus induction was easy for ramie (Boehmeria nivea). MS medium supplemented with 2,4-D or 6-BA alone could effectively induce de-differentiation from cotyledons, hypocotyls, leaves and stem of ramie. Combinations of 6-BA plus an auxin (IAA,IBA,2,4-D,NAA) and combinations of ZT+2,4-D, ZT+IBA, KT+2,4-D and KT+NAA were all effective on callus induction from ramie. The suitable concentration for CTK and AUX were respectively 2.0～2.5 mg/L and 0.2～1.0 mg/L.