盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究

Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde Dehydrogenase Gene under Salt Stress

在NaCl浓度0,3.0,5.0,7.0 g／L下,对转甜菜碱醛脱氢酶(BADH)基因水稻的两个品系51-22,52-7及其受体亲本中花8号进行幼苗耐盐性试验。结果表明:转.BADH基因水稻比其受体亲本耐盐性强,在高NaCl浓度(5.0,7.0 g／L)胁迫下,甜菜碱醛脱氢酶基因可以提高盐胁迫下水稻幼苗过氧化氢酶(CAT)活性,提高根系活力和叶绿素含量,稳定细胞膜的透性,降低植株体内Na+的积累,从而减轻幼苗受盐害的程度。盐胁迫下水稻幼苗的CAT活性、SNa+／K+、叶Na+／K+、叶绿素含量是影响幼苗生长的主要生理指标。

Salt stress experiments of rice seedlings of two lines with a gene encoding for BADH,51 - 22,52 -7 and the recipient Zhonghua No. 8, were conducted under the NaCl concentration of 0,3.0,5.0,7.0 g/L. The results showed that the rice cultivars with a gene encoding for BADH demonstrated higher salt tolerance than its recipient; The gene encoding for BADH could reduce the salt injury by increasing CAT activity, root activity, chlorophyll content and cell membrane osmosis,and by decreasing the Na+ concentration in the seedlings under high NaCl stress (NaCl concentration:5.0,7.0 g/L) ;The CAT activity, SNa*/K+ and chlorophyll content are the main physiological indexes affecting the seedling growth.