白美蓝对兔和大白鼠的胃和食道迷走神经活体染色效果的探讨

LEUCOMETHYLENE BLUE VITAL STAINING ON THE VAGUS NERVE OF THE STOMACH AND OESOPHAGUS IN RABBITS AND RATS

用白美蓝(LMB)液对胃和食道迷走神经进行活体涂染,采用三种还原剂:抗坏血酸、硫代硫酸钠和雕白粉配制的还原型氧化美蓝,在兔和大白鼠的胃和食道迷走神经上进行实验.结果表明:雕白粉-美还原液(RMB)是最合适的神经活体染色剂,染色前用透明质酸酶预处理能促进迷走神经粗大分支着色.

The technique of vital staining on the vagus nerve of stomach and esophagus with leucomethylene blue (LMB) uses three kinds of reducing agents: ascorbic acid,dithioninte and rongaalit methylene blue reduced solutions to experiment on the vagus nerves of stomach and esophagus of rabbits and rats. Rongaalit methylene blue reduced solution is the most suitable vital staining for ne we Preprocessing with hyaluronidase promotes to stain the big branch of vagus nerve.