摘要
以不同PBR322质粒DNA片段作为YAC(yeast artificial chromosome,酵母人工染色体)DNA左右臂探针,使用稀切点限制性内切酶和脉冲电泳(pul-sed field electrophoresis)技术对含有2bA3的800kbYAC作了4种限制酶大尺度物理图谱分析。
Different fragments of plasmid pBR322 DNA were used as the left and right arms probes of YAC (Yeast Artificial Chromosome). The rare cutting restriction enzyme and pulsed field electrophoresis were used for long range physical mapping of YACs. A YAC containing 2bA3 DNA locus with the length of 800 kb was analysed with four different restriction enzyme to obtain the long range physical map.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1993年第4期394-398,共5页
Journal of Fudan University：Natural Science
关键词
YAC
DNA
酵母
大尺度
物理图谱
YAC
pulsed field electrophoresis
2bA3
long range physiscal map
