秋水仙碱对大鼠免疫性肝纤维化中肿瘤坏死因子的影响

Effects of Colchicine on TNF-α in Immuno-hepatic Fibrosis Rats

目的 观察秋水仙碱对肿瘤坏死因子α(TNF -α)的影响。方法 采用猪血清诱导的免疫性大鼠肝纤维化模型 ,ELISA法测定TNF -α血清水平 ,肝组织HE染色后光镜下观察病理变化。结果 秋水仙碱组在给药 (1.0 7×10 -4g/kg) 6周、12周时 ,大鼠TNF -α血清水平均明显低于模型组 (P分别 <0 .0 5、0 .0 1) ;光镜显示肝细胞损伤减轻 ,组织学改善明显。结论 秋水仙碱抑制TNF

Objective To observe the effects of colchicine on TNF-α in immuno-hepatic fibrosis rats.Methods By establishing models of immuno-hepatic fibrosis rats the authors dynamically observed the serum level of TNF-α by ELISA and hepatic tissue pathological changes.Results At administration of colchicine for 6 weeks and 12 weeks,both serum TNF-α level of colchicine group were significantly lower and hepatic tissue pathological changes was much better than those of model group.Conclusion Suppressing the production of TNF-α maybe is one of its anti-hepatic fibrosis mechanisms.