摘要
目的探讨慢性乙型肝炎(CHB)患者血清中白细胞介素-18(IL-18)与病毒载量的关系及意义。方法应用双抗体夹心酶联免疫吸附法(ELISA)、PCR-ELIS法分别检测89例CHB患者血中IL-18和病毒载量。结果①54例病毒载量大于1×104(677.4±1559.1×104)考贝/ml的CHB患者IL-18为240.9±110.0Pg/ml,与对照组(185.5±84.0Pg/ml)相比有显著性差异(P<0.05)。②35例病毒载量小于1×104考贝/ml的CHB患者IL-18为180.0±90.7Pg/ml与对照组相比没有显著性差异(P>0.05)。结论IL-18与CHB的疾病过程相关,可作为CHB患者病毒复制的参考指标。
Objective To explore the relationship and clinical significance between serum interleukin-18(IL-18) and HBV load in patients with chronic hepatitis B(CHB). Methods Serum IL-18 was quantitated by a double antibody sandwich ELISA. HBV DNA was detected by PCR-ELISA. Results (1)In 54 CHB cases with HBV DNA>1×104 copies/ml(677.4±1559.1,×104), IL-18 level was significantly higher(240.9±110.0pg/ml) than that in control group(185.5±84.0pg/ml,P<0.05). (2)In 35 CHB cases with HBV DNA<1×104 copies/ml, IL-18 level was 180±90.7pg/ml. There was no sighificant difference between the CHB and control group(P>0.05). Conclusion It suggests that IL-18 is involved in the process of CHB and that can be used as a marker of HBV replication.
出处
《江西医学检验》
2004年第3期213-214,共2页
Jiangxi Journal of Medical Laboratory Sciences
