几种吡咯里西啶类生物碱对肝细胞毒性的探讨

Toxicity of Several Pyrrolizidine Alkaloids on Hepatocytes

目的 :考察几种吡咯里西啶类生物碱体外实验中对肝细胞的毒性。方法 :用MTT法检测了 6种吡咯里西啶类生物碱clivorine ,monocrotaline ,isoline ,LP 0 1(yamataimine) ,LP 0 2 (12 acetylyamataimine) ,LJ 0 9(platy phylline)对人正常肝细胞株L 0 2细胞活性的影响 ,对于其中有毒性的clivorine又用结晶紫染色的方法进一步确证其毒性。结果 :Clivorine对肝细胞具有强烈的毒性作用 ,未发现其它几种吡咯里西啶生物碱对肝细胞的毒性。结论

AIM: To test the toxic effects of clivorine,monocrotaline,isoline,platyphylline,yamataimine and 12-acetyl yamataimine pyrrolizidine alkaloids isolated from Ligularia and Monocrotalia on hepatocytes.METHOD: Using MTT experiment to test the effect of these pyrrolizidine alkaloids on hepatocytes.Using crystal violet staining assay to make a further validation of the toxic effect of clivorine.RESULT:Clivorine had toxic effect on hepatocytes and other pyrrolizidine alkaloids had no effect on hepatocytes.The crystal violet staining assay confirmed clivorine's toxic effect.CONCLUSION:Clivorine has direct toxic effect on hepatocytes.