喉癌中人类端粒酶逆转录酶的表达

The Expression of HTRT in Laryngeal Cancer

目的探讨人类端粒酶逆转录酶(human telomerase reverse transeriptase,HTRT)在喉癌中的表达及其在肿瘤中的可能作用。方法运用免疫组化链菌素-生物素标记法(Lablled streptavidin-biotin,LSAB)检测60例喉癌和20例喉正常黏膜中 HTRT 的表达。结果 60例喉癌中 HTRT 阳性表达率为78.3%,20例癌旁组织中HTRT 阳性表达率为15%,20例喉正常黏膜中全部为阴性。喉癌中 HTRT 阳性表达率明显高于喉正常黏膜,差异有显著性。HTRT 表达与肿瘤分化程度,临床分型及淋巴结转移不相关。结论 HTRT 表达在喉癌发生中起一定作用,可作为判断肿瘤生物行为的一个有用指标。

Purpose In order to investigate the genic activity of human telomerase reverse transcriptase(HTRT)in laryngeal cancer and its possible role.Methods The expression of HTRT in 60 cases of laryngeal carcinoma and 20 cases of normal laryngeal mucosa was studied with LSAB to inmunohistochemical method.Results HTRT was negative in all of the 20 normal laryngeal mucosa specimens,but positive in 78.3%(47/60)of the laryngeal carinoma specimens.A significantly higher positive rate was found in laryngeal carcinoma than in normal laryngeal mucosa.The expression of HTRT did not correlate with histological grading,tumor stage and lymph node metastasis.Conclusion HTRT may play an important role in the process of carcinogenesis in laryngeal mucosa and be used as a useful marker for the assessment of biological behavior of tumors.