p27在慢性粒细胞白血病中表达的研究

THE STUDY OF THEEXPRESSI ON OF p27 IN CML

目的:研究细胞周期蛋白依赖激酶抑制物p27在慢性粒细胞白血病(CML)患者中的表达,探讨p27在CML中的作用机制及其临床意义。方法:应用蛋白印迹(Western蛳blot)(DAB显色与化学发光显示)法检测27例CML患者及10例对照组p27的表达,分析p27在CML中有无异常表达及其表达与病情进展和预后的关系。结果:27例CML患者化学发光法显示p27阳性表达率(37.04 %)明显低于对照组(80.00 %)(P<0.05)。在27例CML患者中,p27阳性表达率在高危、中危、低危3组中分别为0、28.57 %、44.44 %;高危组与低危组间有明显差异(P<0.05),而高危组与中危组、中危组与低危组间无差异。化学发光法结果优于DAB显色法。结论:p27在CML中有异常低表达,提示p27在CML中有抑癌基因的作用,其表达水平可能与CML患者的病情进展及预后相关。

Objective:Thepurposeistostudytheexpressionofp27inpatientswithchroicmyelogenousleukemia(CML),andevaluatethefunctionandclinicalvalueofp27inchronicleukemia.Methods:Theexpres-sionofp27wasmeasuredinthebonemarrowsamplesof27patientswithCMLand10casesincontrolgroupwereexaminedbywestern蛳blotmethod(DABmethodandchemicalphotoflurography).Andanalysetherelationshipbetweentheexpressionlevelofp27andtheprogressionandprognosisofpatientswithCML.Results:Thepositiverateofp27in27CMLpatientswere37.04%,whichwasapparentlylowerthanthecontrolgroupwithrateof80%,therewassignificantdifferencebetweenpatientswithCMLandcontrolgroup(P<0.05),andthepositiveexpressionofp27inhighriskgroup(0)waslowerthanthatinlowriskgroup(38.8%)ofCMLpatients,thedifferencebetweenthemwasapparent(P <0.05).theresultbychemicalphotoflurographyisbetterthanonebyDABmethod.Conclusion:p27hadlowexpressioninleukemiapa-tients,Ourresultssuggestpotentialrolesofp27inleukemiamightbeananti蛳oncogene,andtheexpressionlevelofp27isprobablycorrelatedwiththeprogressionandprognosisofpatientswithCML.