脊神经阻滞对妊娠高血压综合征大鼠24h尿蛋白的影响

Effect of spinal nerve block on the 24-hour urine protein in rats with pregnancy-induced hypertension

目的:探讨连续脊神经阻滞对妊娠高血压综合征(简称妊高征)大鼠的蛋白尿具有改善的作用。方法:选择健康雌性Wister怀孕大鼠30只随机分成3组,每组10只,在孕13d实验组给予甲基左旋精胺酸甲酯(50mg/d),治疗组手术行硬膜外置管,每日给1.25g/L布比卡因25μL,同时给予甲基左旋精胺酸甲酯(50mg/d)。采用磺柳酸法测定各组13,20d24h尿蛋白总量。实验结果采用成组设计t检验进行统计分析。结果:在孕13d对照组24h尿蛋白总量(7.47±1.74)mg,实验组(7.48±4.45)mg,治疗组(97.65±1.81)mg,各组间差异无显著性意义(P>0.05)。在孕20d对照组24h尿蛋白总量(10.05±3.16)mg,实验组(94.05±20.10)mg,治疗组(32.06±8.18)mg,实验组、治疗组均较对照组明显升高(P<0.01),治疗组较实验组明显降低(P<0.01)。结论:连续脊神经阻滞对妊高征大鼠的蛋白尿具有明显改善作用。

AIM:To study the effect of continuous spinal nerve block in improving proteinuria of pregnancy induced hypertension(PIH) rats. METHODS:Thirty healthy female Wister pregnant rats were randomly divided into control,experiment and treatment groups with 10 rats in each group.L NAME(50 mg per day) was given to experiment group on the 13th pregnant day.Rats in the treatment group were preformed with epidural intubation and were given 1.25 g/L bupivacaine 25 μL per day and L NAME(50 mg per day) simultaneously.24 hour total urine protein of each group on the 13th and 20th day were measured by means of sulphur willow acid.The results were statistically analyzed by using inter group t test . RESULTS:On the pregnant 13th day,24 hour total urine protein in the control group,experiment group and treatment group were (7.47±1.74) mg,(7.48±4.45) mg and (7.65±1.81) mg,respectively.There was no statistical difference among the three groups(P >0.05).On the pregnant 20th day,24 hour total urine protein in the control group,experiment group and treatment group were (10.05±3.16) mg,(94.05±20.10) mg and (32.06±8.18) mg.It was obviously increased in the experiment group and treatment group,as compared with the control group (P< 0.01),and much lower in the treatment group than in the experiment group(P< 0.01). CONCLUSION:Continuous spinal nerve block can obviously improve proteinuria in rats with PIH.