高效液相色谱法测定益肾健骨片中齐墩果酸的含量

Determination of Oleanolic Acid in Yishen Jiangu Tablets by HPLC

目的 :建立 HPL C测定益肾健骨片中齐墩果酸含量的方法。方法 :色谱条件为 :C1 8柱 ,流动相为乙腈 - 0 .1%磷酸 (6 7∶ 33) ,UV检测波长为 2 10 nm。结果 :此方法线性关系良好 ,平均加样回收率为 97.2 5 % (n=6 )。结论 :该方法分离良好 ,结果准确可靠。

Objective:To establish the determination method of Oleanolic acid in Yishen Jiangu Tablets.Methods:The HPLC method for determination was:C 18 column(150 mm×4.6 mm,5 μm),mobile phase:acetonitrile-0.1% phosphoric acid (67∶33),detection wavelength:210 nm.Results:This method showed a good linear relationship.The average recovery was 97.25%,RSD=0.38%(n=6).Conclusion:This method showed a good separating degree and reliable.