CCR9在T淋巴细胞白血病CD4^+T细胞上表达的变化及临床意义

The clinical significance of the expression of CCR9 on the CD4^+T cells of T-ALL and T-CLL

目的 :探讨淋巴细胞白血病 (TLL)CD4 + T细胞上CCR9表达的变化及临床意义。方法 :选择 38例TLL患者 [T 急性淋巴细胞白血病 (ALL) 2 1例 ,T 慢性淋巴细胞白血病 (CLL) 17例 ],对照组 18例。用免疫磁珠分选出各组CD4 + T细胞 ,以流式细胞仪分别测定CCR9在T ALL和T CLLCD4 + T细胞上的表达及在对照组CD4 + T细胞上的表达 ,各组间进行对比分析。结果 :CCR9表达在T ALL与T CLL患者CD4 + T细胞比较 [(73± 17) %∶(35± 18) % ,P <0 .0 1],与对照组 (2 .0± 1.0 ) %比较差异有统计学意义 (P <0 .0 1)。结论 :TLL患者CD4 + T细胞存在CCR9高表达 ,CCR9检测可能作为其诊断和疗效观察指标之一。

Objective:To study changes and the clinical significance of expression of CCR9 on the CD4+T cells of T-ALL and T-CLL.Method:38 patients with TLL(T-ALL 21 cases, T-CLL 17 cases) were preformed and divided into two groups: T-ALL group and T-CLL group, There were 18 cases in control group. CD4+T cells in each group were isolated by immuno-magnetic bead. Subsequently, Flow-cytometry was used to detect the expression of CCR9 on the CD4+T cells in each group. Then three groups were compared each other to assess.Result:Compared to the T-CLL, the expression of CCR9 on T-ALL CD4+T cells increased significantly(73±17%VS 35±18%, P< 0.01), and was much higher than that of control group (73±17% VS 2.0± 1.0%, P< 0.01). The expression of CCR9 on T-CLL CD4+T was elevated much more than that of control group ( 35±18%VS 2.0±1.0%,P< 0.01).Conclusion:The altered expression of CCR9 can be one of the targets that evaluate the effect of therapy and prognosis for T-ALL or T-CLL and distinguish T-ALL from T-CLL.